<p>Binding of different concentrations of OsMYB55 protein to 200 ng of DNA containing one copy of the MB-box (<b>A</b>). Transcription activation was carried out by using GUS based transient assay and 4-methylumbelliferyl b-Dglucuronide (MUG) as the substrate (<b>B</b>). The 4-methyl umbelliferone (MU) was used to generate the standard curve. Bars represent mean ± SE. (n = 6).</p
The identification of Myb 'target' genes will not only aid in the understanding of how overexpressio...
<p>Electrophoretic mobility shift assay (EMSA) with Jurkat and H441 nuclear extracts was performed i...
A. Nucleotide frequency distribution of the OsMADS25 core binding consensus sequence as determined C...
<p>(<b>A</b>) The sequences of the cdODN-SOC carrying <i>cis</i>-elements for Sox2, Oct4 and c-Myc a...
<p>(A) DNA probes used in GMSA. Closed triangles indicate TGACGT sequences. (B) GMSA was performed u...
A. Schematic diagram of OsP5CR promoter region showing the CArG–box motifs. B. Electrophoretic mobil...
In this work, several major procedures of the electrophoretic mobility shift assay (EMSA) were modif...
Electromobility shift assay (EMSA) depicting binding of unphosphorylated (A) and in vitro phosphoryl...
The electrophoretic mobility shift assay (EMSA), also known as “gel shift assay”, is used to examine...
<p>(<b>A</b>) Overview of oligonucleotides used for EMSA. LKB1 DSE: downstream element, position +35...
<p>(a) Lane 1, <sup>32</sup>P-labeled MSgt-FSgt probe only; lane 2–3, <sup>32</sup>P-labeled MSgt-FS...
<p>(A) Competitive EMSAs were performed with <i>P3</i> (left) and <i>P5</i> (right) probes in NE of ...
<p>a) Comparison of expression levels from <i>GSK3B</i> promoter constructs by luciferase reporter g...
Confirming the binding of a transcription factor with a particular DNA sequence may be important in ...
<p>(<b>A</b>) A positive result (band shift) was observed with all the tested FA genes. Lane 1: cont...
The identification of Myb 'target' genes will not only aid in the understanding of how overexpressio...
<p>Electrophoretic mobility shift assay (EMSA) with Jurkat and H441 nuclear extracts was performed i...
A. Nucleotide frequency distribution of the OsMADS25 core binding consensus sequence as determined C...
<p>(<b>A</b>) The sequences of the cdODN-SOC carrying <i>cis</i>-elements for Sox2, Oct4 and c-Myc a...
<p>(A) DNA probes used in GMSA. Closed triangles indicate TGACGT sequences. (B) GMSA was performed u...
A. Schematic diagram of OsP5CR promoter region showing the CArG–box motifs. B. Electrophoretic mobil...
In this work, several major procedures of the electrophoretic mobility shift assay (EMSA) were modif...
Electromobility shift assay (EMSA) depicting binding of unphosphorylated (A) and in vitro phosphoryl...
The electrophoretic mobility shift assay (EMSA), also known as “gel shift assay”, is used to examine...
<p>(<b>A</b>) Overview of oligonucleotides used for EMSA. LKB1 DSE: downstream element, position +35...
<p>(a) Lane 1, <sup>32</sup>P-labeled MSgt-FSgt probe only; lane 2–3, <sup>32</sup>P-labeled MSgt-FS...
<p>(A) Competitive EMSAs were performed with <i>P3</i> (left) and <i>P5</i> (right) probes in NE of ...
<p>a) Comparison of expression levels from <i>GSK3B</i> promoter constructs by luciferase reporter g...
Confirming the binding of a transcription factor with a particular DNA sequence may be important in ...
<p>(<b>A</b>) A positive result (band shift) was observed with all the tested FA genes. Lane 1: cont...
The identification of Myb 'target' genes will not only aid in the understanding of how overexpressio...
<p>Electrophoretic mobility shift assay (EMSA) with Jurkat and H441 nuclear extracts was performed i...
A. Nucleotide frequency distribution of the OsMADS25 core binding consensus sequence as determined C...