Mast cell degranulation results in extracellular mitochondrial particle secretion.

<p>(A) LAD2 cells were stained with MitoTracker and LysoTracker as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049767#pone-0049767-g001" target="_blank">Fig. 1</a>. mitochondria distribution was observed in resting (upper panels) and degranulated (bottom panels) mast cells stimulated as shown. The left panels depict secretory granules in green and the middle panels represent mitochondria fluorescence in red. The right panels represent images merged from the two previous panels. The lower set of three panels show cells in lower magnification as indicated. White rectangles in the middle panels indicate extracellular mitochondriaL particles stained by MitoTracker. Supernatant fluids from both stimulated and control LAD2 cells were collected and assayed for (B, C) mt-7s and mt-CytB, as well as (D) ATP (n = 3; *p<0.05, **p<0.01 compared to control). Sup = Supernatant fluid.