Knockdown of RNA binding proteins in MCF-7 cells and their effect on the mRNA levels of Mcl-1 splice isoforms.

<p>MCF-7 cells were transfected with SRSF1-6, Tra2β, SREK1, GAPDH (G) and Negative control (NC) siRNAs, or treated with vehicle (lipid) only (V), or were left untreated (UT). (A) Semi-quantitative RT-PCR showing both Mcl-1 spliced variants. (<b>B</b>) Semi-quantitative RT-PCR showing knockdown of RNA binding proteins 48 hours after transfection with siRNAs. (<b>C</b>) Semi-quantitative RT-PCR showing levels of the Mcl-1 splice isoforms (Mcl-1_L and Mcl-1_S) and the loading control GAPDH 72 hours after transfection with siRNAs. (<b>D</b>) Mcl-1_L levels measured by real-time PCR on the same sample shown in (B). (<b>E</b>) Mcl-1_S levels measured by real-time PCR on the same sample shown in (B). (<b>F</b> Mcl-1_S levels in sample replicates measured by real-time PCR (mean (n = 3) ± SEM). ** P≤0.01; * P≤0.01.