Ets proteins are involved in VEGFR-3 expression and VEGFR signaling.

<p>A. Western blots of phosphorylated Ets1 (pEts1), Ets1 and total Ras proteins in immortalized mouse LECs (mLECs) transfected with control siRNA or mixed siRNAs for <i>ras</i> genes (<i>Hras</i>, <i>Nras</i> and <i>Kras</i>) in the presence or absence of VEGF-A, VEGF-C or VEGF-D. B. Real-time RT-PCR assay for mRNAs in mLECs transfected with control, Ets1 and Ets2 siRNAs. si-1 and -2 represent two individual siRNAs. Ets1/2 si represents transfection with mixed siRNAs for Ets1 and Ets2. Error bars represent the S.D.; n = 3. *p<0.05, ***p<0.005, ****p<0.001 (vs. mLECs transfected with control siRNA; see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051639#pone.0051639.s006" target="_blank">Table S2</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051639#pone.0051639.s007" target="_blank">Table S3</a>). C. Protein expression in mLECs transfected with control, Ets1 and Ets2 siRNAs. Left panel, western blots; right panel, quantitative analysis of western blots. Error bars represent the S.D.; n = 3. **p<0.01, ***p<0.005, ****p<0.001 (vs. mLECs transfected with control siRNA).