Human ETS1 is involved in <i>VEGFR3</i> gene expression, cell proliferation and network formation by human LECs.

<p>A. Real-time RT-PCR assay for <i>VEGFR3</i> mRNA in primary human LECs (hLECs) transfected with control and ETS1 siRNAs. si-1 and -2 represent two individual siRNAs. Error bars represent the S.D.; n = 3. ***p<0.005, ****p<0.001 (vs. hLECs transfected with control siRNA; see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051639#pone.0051639.s010" target="_blank">Table S6</a>). B. WST-1 assays and BrdU assays using ETS1-knockdown hLECs. Error bars represent the S.D.; n = 12. ***p<0.005, ****p<0.001 (vs. hLECs transfected with control siRNA). C. DiI-stained cellular networks of hLECs transfected with control and ETS1 siRNAs on Matrigel. Scale bar = 500 µm. DiI-labeled areas were quantified and the mean area of DiI-labeled wild-type hLECs was normalized to 1. Error bars represent the S.D.; n = 3. *p<0.05, ****p<0.001 (vs. hLECs transfected with control siRNA).