The proliferation-refractory phenotype of CD8⁺ T cells cross-primed TERS-imprinted BMDC can be rescued by excess antigen or L-norvaline, but not by addition of IL-2.

<p>BMDC were co-cultured with OT-I CD8⁺ T cells as in Fig. 4. (<b>A</b>) CD8⁺ OT-I T cells were co-cultured with SIINFEKL-pulsed TERS-imprinted BMDC and CD8⁺ T cell proliferation was measured by CFDA-SE dilution. Results are representative of four independent experiments. (<b>B</b>) Recombinant mouse IL-2 was added at 30 or 100 U/mL to the co-cultures as indicated, and CD8⁺ T cell proliferation was measured by CFDA-SE dilution. Results are representative of two independent experiments. (<b>C</b>) After 4-day co-culture, CFDA-SE-labeled CD8⁺ T cells were recovered and rested for 2 days before restimulation with SIINFEKL-pulsed BMDC, with or without exogenous rmIL-2 (30 U/mL). CD8⁺ T cell proliferation was measured by CFDA-SE dilution. Results are representative of two independent experiments. (<b>D</b>) L-arginine (L-arg, 2 mM) or L-norvaline (L-nor, 10 mM) was added to co-cultures and CD8⁺ T cell proliferation was measured by CFDA-SE dilution. Results are representative of four independent experiments. (<b>E</b>) BMDC with (+) and without (−) OVA were co-cultured with CFDA-SE-labeled CD8⁺ OT-I T cells at a 1∶1∶2.5 ratio as above and T cell proliferation was measured by CFDA-SE dilution. Results are representative of four independent experiments.