Transcriptional and phenotypic analysis of CD8 T cells cross-primed by TERS-imprinted BMDC.

<p>(<b>A</b>) After 96-hr co-culture, CFDA-SE-labeled CD8⁺ T cells were purified, the mRNA isolated and analyzed by RT-qPCR for transcription levels of the indicated genes. Columns indicate fold increase in transcript level (RQ) of each treatment group. An Unstim (+) control was set arbitrarily to 1. Error bars represent SEM of two biological replicates and are representative of four independent experiments. *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001, ns = not significant, unpaired, two-tailed <i>t</i> test. FOXP3 expression was interrogated by intracellular flow cytometry. (<b>B</b>) After 96 hr co-culture, CD8⁺ T cells were interrogated for CD28 and LAG3 surface expression by flow cytometry. Results are representative of three independent experiments. (<b>C</b>) Supernatants from 96 hr co-cultures were interrogated for the presence of cytokines using the BD® Cytometric Bead Array assay. Results are pooled from two independent experiments.