RCCS aggregates and 2D monolayers exhibit distinct metabolic profiles.

<p><b>A –</b> Heatmap dendrogram of the HR-MAS metabolic profiles generated for KNS42, U87 and PFSK1 cell lines grown in 2D and 3D, showing clustering together of replicates grown by the same culture method with metabolites on the x-axis and samples on the y-axis. <b>B –</b> Principal components analysis of the HR-MAS data demonstrates further clear clustering by cell type and culture method. <b>C –</b> Barplot of metabolite levels for the three cell lines, comparing 2D and 3D culture averaged across three replicates for each sample with standard error bars, demonstrating significant differences for many metabolites between culture methods with consistent increases in lipids, myo-inositol and glycine and decreased phosphocholine in 3D for all cell lines.