Characterization of inflammatory cell infiltrates in dorsal skin of WT and in Ctip2^ep−/− adult mice.

<p>(<b>A</b>) Combined eosinophil and mast cell (C.E.M) staining for eosinophils (pink) and mast cells (blue). Black arrows point to eosinophils. Scale bar: 50 µm. (<b>B</b>) Immunohistochemical staining of dorsal skin biopsies from WT and Ctip2^ep−/− mice were performed with specific antibodies against CD3 (red). Yellow arrowhead indicates dermal infiltrates of CD3+ cells. Scale bar: 100 µm. (<b>C</b>) Percent CD3+ T cells at 2 m and 4 m. (<b>D</b>) Immunostaining of CD4+ T cells (red) in WT and mutant mice. Scale bar: 100 µm. (<b>E</b>) Percent CD4+ T cells at 2 m and 4 m. All sections were counterstained with DAPI (blue). Statistical analyses were performed by student's unpaired t-test using GraphPad Prism software; ** P<0.005.