RNS60 inhibits infiltration of mononuclear cells and inflammation in the CNS of EAE mice.

<p>Cerebellar (A) and spinal cord (B) sections of control EAE (16 dpt), and either RNS60− or NS-treated EAE mice (16 dpt receiving RNS60/NS from 8 dpt) were stained with H & E. Digital images were collected under bright field setting using a 40× objective. Infiltration (C) and cuffed vessel (D) were represented quantitatively by using a scale as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051869#s2" target="_blank">materials and methods</a>. Data are expressed as the mean ± SEM of five different mice. <i>^ap<0.001</i> vs control; <i>^bp<0.001</i> vs EAE. Cerebellum of control, EAE and either RNS60− or NS-treated EAE mice was analyzed for iNOS and IL-1β by semi-quantitative RT-PCR (E) and quantitative real-time PCR (F). Data are expressed as the mean ± SEM of five different mice. <i>^ap<0.001</i> vs control; <i>^bp<0.001</i> vs EAE.