Quantitative data analysis demonstrates the translational status of the TGFβ-induced post-transcriptional EMT gene signature.

<p>(A) Schematic of polysome profile analysis. Monosomal fractions (M; 40S, 60S, and 80S fractions) and polysomal fractions (P) from NMuMG or E1KD cells treated ± TGFβ for 24 hr were isolated by sucrose gradient centrifugation and pooled. (B) Heatmap of the raw signal intensity values of the differential gene expression profile for the EMT signature genes compared to total, unfractionated mRNA. (C) RT-PCR analysis of microarray RNA samples was used to demonstrate the differential gene expression profile of ILEI.