Atherosclerotic lesion measurement in aortic <i>en face</i> preparations and aortic root cross sections.

<p>The images show representative <i>en face</i> (left) and aortic root cross-section preparations (right) from apoE^−/− (A) or LDLr^−/− (B) mice. Mice were fed HFC diet with or without supplementation for 11 weeks as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052634#pone-0052634-g001" target="_blank">Figure 1</a>. Quantification of percent lesion area in the <i>en face</i> preparations was calculated relative to total aorta area. Quantification of cross-sectional area occupied by lesion in the aortic root was determined relative to total cross-sectional area. * vs. HFC, p<0.05; ** vs. HFC, p<0.01.