ATP-induced shedding of CD62L in CD4⁺, CD8⁺ and DN T lymphocytes expressing the transmembrane tyrosine phosphatase B220.

<p>Spleen cells from 3 to 5-mo-old MRL<i>^+/+</i>, MRL<i>/lpr</i>, B6 and B6/<i>lpr</i> mice (n = 5 mice/group) were either left unstimulated (□) or stimulated with 500 µM ATP (▪) for 45 min at 37°C. Spleen cells were then stained with anti-CD90, anti-B220, anti-CD62L and anti-CD4 or anti-CD8 mAb to assess CD62L expression on B220<i>^–</i> and B220⁺ CD90⁺ T cells (either CD4⁺ or CD8⁺) from MRL<i>^+/+</i>, MRL<i>/lpr</i>, B6 and B6/<i>lpr</i> mice as well as on B220⁺ DN CD90⁺ T cells from MRL<i>/lpr</i> and B6/<i>lpr</i> mice. Asterisks denote statistically significant differences between ATP-stimulated and unstimulated groups: **<i>p</i>≤0.01; ***<i>p</i>≤0.001.