¹³C-Profiles of amino acids from host cells cultivated in the presence of [U-¹³C₆]glucose.

<p>(A) ¹³C-Excess (mol %; displayed as a color code) of amino acids derived from BMM cells; for each condition, two independent labeling experiments were performed. The color for each amino acid correlates with the mean value of three MS determinations with the same sample. (B) ¹³C-Excess (mol %; displayed as a color code) of amino acids derived from J774A.1 cells; for each condition, two independent labeling experiments were performed. The color for each amino acid correlates with the mean value of three MS determinations with the same sample. (C) Relative isotopologue composition of amino acids derived from BMM. Colored columns indicate the % amounts of isotopologues comprising 1, 2, 3, 4, or 5 ¹³C-atoms (M+1 to M+5; left scale; * indicate values from only one labeling experiment; otherwise, the values represent means from two labeling experiments). ¹³C-Excess values are shown as grey columns (mol %, right scale; error bars indicate SD for two independent labeling experiments). (D) Relative isotopologue composition of amino acids derived from J774A.1 cells. Colored columns indicate the % amounts of isotopologues comprising 1, 2, 3, 4, or 5 ¹³C-atoms (M+1 to M+5; left scale; the values represent means from two labeling experiments). ¹³C-Excess values are shown as grey columns (mol %, right scale; means from two independent labeling experiments; error bars indicate SD for the two labeling experiments). Experiments were done with and without infection by <i>Listeria monocytogenes</i> (<i>L.m.</i>) and with (+) and without (-) activation by interferon-γ (IFN-γ). The color maps indicate ¹³C-excess in semi-logarithmic form to show even relatively small ¹³C excess values; for numerical values, see also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052378#pone.0052378.s003" target="_blank">Table S1</a>.