Immunofluorescence detection of ZO-1 and Na/K ATPase α1 in HCEnC-21 and HCEnC-21T.

<p>(A–C) Confluent monolayers of passage-13 HCEnC-21 (A) passage-14 HCEnC-21T (B) and passage-3 primary stromal fibroblasts (C) were fixed and labeled for ZO-1 (green) and nuclei (blue). (D–F) Confluent monolayers of HCEnC-21 (D), HCEnC-21T (E), and primary stromal fibroblasts (F) were fixed and labeled for Na/K ATPase α1 (green) and nuclei (blue). Note that ZO-1 and Na/K ATPase α1 were detected in HCEnC-21 and HCEnC-21T but not in stromal fibroblasts, and localized to the cell-cell contacts and plasma membrane, respectively. 400x.