G460W adducin influences CFTR trafficking: FRAP experiments.

<p>A) Cell images from a FRAP experiment: YFP-CFTR signal in a HEK293 T cell over-expressing CFP-WT adducin (<i>Add-WT</i>, upper panels) or CFP-G460W adducin (<i>Add-G460W</i>, lower panels) before YFP photobleaching (<i>pre PB</i>) and at different time-points after the photobleaching (<i>post PB t = 0 s, 20 s, 50 s</i>); on the right the corresponding CFP-adducin signal (<i>Add</i>) is reported for each condition. In the pre-photobleaching images three membrane ROIs are shown. Scale bar is 5 µm. The graph shows the kinetics of CFTR-YFP fluorescence recovery after photobleaching in presence of the CFP-adducin WT or G460W mutated variant. B) Mean CFTR mobile fraction percentage (F_mob%) and mean half-life constants (t_1/2) of HEK cells over-expressing YFP-CFTR and CFP-WT adducin (n = 38) or CFP-G460W adducin (n = 42). *P<0.05.