Effect of WT and mutant E proteins on the expression of prM protein.

<p>293T cells were transfected with prM construct alone or prM construct and increasing amounts (0 to 2 µg) of WT E construct (A), E construct without the stem and anchor, Ed395 (B) or CD4D4SA construct containing the stem and anchor of E fused to the ectodomain of CD4 (C) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052600#pone.0052600-Hsieh1" target="_blank">[31]</a>. Cell lysates collected at 48 h post-transfection were subjected to Western blot analysis using human serum of a confirmed dengue case <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052600#pone.0052600-Hsieh1" target="_blank">[31]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052600#pone.0052600-Wang1" target="_blank">[32]</a>. One representative experiment of three is shown. The size of molecular weight markers is shown in kDa. Arrow heads indicate prM, E, Ed395 or CD4D4SA protein.