CD4⁺CD25⁺-induced inhibition of target T-cell proliferation is cytokine-dependent.

<p>CD4⁺CD25⁺ T cells were incubated with IL-2 (200 U/ml), HSP70 (10 µg/ml) or both for 2 h, washed and co-cultured with CFSE-labeled CD4⁺CD25⁻ target T cells (E∶T ratio 1∶10) on 96-well plates coated with anti-CD3 antibodies (OKT3, 1 µg/ml) in serum-free medium. For control CD4⁺CD25⁻ target T cells were culture in the absence of Tregs. After 5 days of incubation, the supernatants were analyzed for (<b>A</b>) IL-10, (<b>B</b>) TGF-β, (<b>C</b>) IFN-γ and (<b>D</b>) TNF-α. Results of six independent experiments, expressed as mean ± SD. p-values (* p<0.05, ** p<0.01 or ***p<0.001) are indicated with asterisks.