Solid phase analysis of WARP/collagen VI interaction.

<p>a, WARP binding to collagen VI tetramers. Coated collagen VI was incubated with recombinant WARP. Data are means of triplicate determinations +/− SE. <i>Inset</i>, Scatchard analysis of binding data reveals an apparent kDa of 22 nM. b, Reciprocal solid phase binding experiment showing collagen VI binding to coated WARP dimer but not WARP multimers. Representative curves are shown. c, WARP binds to both pepsinized collagen VI tetramers (black diamonds) and pepsinized intact collagen VI (white squares).