FIX-ΔLUNA virus does not increase viral genome copy number after IL6 induced differentiation of CD14⁺ cells.

<p>Viral DNA was quantified by qPCR. A) Total DNA was extracted from FIX-WT, FIX-ΔLUNA, and FIX-Rev infected HF cells over a 20 d time course. B) Total DNA was extracted from FIX-WT, FIX-ΔLUNA, and FIX-Rev infected CD14⁺ cells at 1 dpi, 3 dpi, 5 dpi, 10 dpi, 20 dpi. Differentiation was induced in CD14⁺ cells at 10 dpi by the addition of IL6 and at 5 days (sample termed as 15dpIL6) or 10 days (samples termed as 20dpIL6) total DNA was collected. All samples were run in triplicate. Statistical analysis was done using a Student <i>t</i> test comparing FIX-WT vs. FIXΔLUNA or FIX-Rev vs. FIX-ΔLUNA. The * indicates a <i>P</i>-value<0.01, and the ** indicates a <i>P</i>-value<0.001.