Evaluation of Ag-specific T cell stimulatory capacity of APCs.

<p>Ag-specific T cell proliferative responses were evaluated by [³H] thymidine incorporation. (A) OVA-specific CD4 T cells, and (B) PLP_180–199-specific CD4 T cells from NLC mice were co-cultured with T cell-depleted/mitomycin C-treated/OVA pulsed APCs (A) and PLP_180–199 pulsed APCs (B). Open bars are Tg-<i>Kif1c^D2</i>-APCs, and closed bars are NLC-APCs. Each bar represents the mean cpm ± SEM of 3 independent experiments. The significance of the differences was determined by two-way ANOVA. OVA-specific response: effect of [OVA] (p<0.0001); effect of strain (p<0.0001); interaction (p = 0.08). PLP_180–199-specific response: effect of [PLP_180–199] (p<0.0001); effect of strain (p<0.0001); interaction (p = 0.08).