Percentage mass change throughout embryonic development for different constituents.

<p>Relationship between embryo total length (TL) and (a) total wet mass (n = 82), (b) total organic matter (<i>n</i> = 81), (c) total inorganic matter (<i>n</i> = 81) and (d) total water content (<i>n</i> = 82) of <i>Heterodontus portusjacksoni</i> embryonic system at different developmental stages; the <i>solid line</i> represents the eviscerated embryo mass (without external and internal yolk sacs) and embryo TL relationship; the <i>parallel lines</i> were designed to include all the data points, following the proposed method by Guallart and Vicent <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0050196#pone.0050196-Guallart1" target="_blank">[15]</a> and the intersection of the <i>parallel lines</i> with the eviscerated embryo mass–TL relationship indicates the mass change corresponding to the extremes of variability of egg size.