Biomarker profiles of rbST-treated (left) and untreated (right) dairy cows.

<p>Profiles from animal study I (dotted lines) and animal study II (solid lines) are shown. Sera from adaption period (3 sera from every cow), treatment period (13 sera per cow from animal study I and 9 sera per cow from animal study II) and withdrawal period (5 sera per cow from animal study I and 6 sera per cow from animal study II) were measured in duplicate. Biomarkers shown are concentrations of IGF-1 (A), B/B0 levels of IGFBP2 (B), B/Bd levels of antibodies against rbST (C) and concentrations of osteocalcin (D). The rbST treatment schedules for both animal studies are indicated by two black horizontal bars and decision limit per biomarker by the green horizontal line. Note that cows from animal study II received two additional rbST injections after the biweekly treatment period.