E2 potentiates the inhibiting effect of n-3 PUFAs on BCa cell growth.

<p><b>A</b>, Dose response of DHA or EPA in MCF-7 cells with or without E2. The cells were treated with indicated concentrations of n-3 PUFAs for 72 hours ± E2 and cell growth was evaluated by MTT assay (see Material and Methods). Data were normalized to the percent of cell growth in control wells. (n = 5). <b>B</b>, Time course for n-3 PUFAs effects in MCF-7 cells. MCF-7 cells were pretreated with 90 µM DHA or EPA with or without 10 nM E2 for different time points (as indicated). Cell growth was measured with the MTT assay (n = 5). <b>C</b>, Time course for n-3 PUFAs effects in T47D cells. T47D cells were treated and followed with MTT assay as the same in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052838#pone-0052838-g001" target="_blank">Figure 1 B</a>. (n = 4). <b>D</b>, Quantitated data from Anchorage-Independent Growth Assay (see Methods, n = 3). **p<0.001.