Expression of lytic molecules by IL-15 DCs.

<p>(A) Matured CD56⁺ IL-15 DCs were analyzed by flow cytometry for cell surface expression of TNF-α, FasL and TRAIL (solid line histograms). Filled grey histograms represent isotype controls. Data are from one experiment representative of three. (B) Both CD56⁺ (solid line) and CD56⁻ (dashed line) IL-15 DCs were stained for intracellular expression of TRAIL, granzyme B and perforin. Filled grey histograms represent isotype controls. One representative experiment out of 6 (for TRAIL) and 9 (for granzyme B and perforin) is shown. (C) Supernatants from overnight washout cultures of CD56⁺ (▪) and CD56⁻ (□) IL-15 DCs were analyzed for granzyme B release by ELISA. Bars represent mean (± SEM) granzyme B concentations (pg/mL) from 7 experiments.*, <i>P</i><0.05.