Effect of NO on cell viability and β-catenin mediated gene expression A–D.

<p>NO effect on β-catenin mediated gene expression of NFκB and Wnt targets. H5V confluent monolayers were incubated with IFNγ/LPS, IFNγ/LPS/LNMMA, SNAP or NAP for 12 and 24 h. RNA was extracted from the cells and transcript levels of the antiapoptotic molecule A20 (panel A), iNOS (panel B), VE-cadherin (panel C) and survivin (panel D) quantified by RT-PCR. All values are normalised to GAPDH values and expressed as Fold to the 0 h stimulation value. <b>E.</b> NO does not affect the viability of H5V cells. H5V cells were treated with IFNγ/LPS or IFNγ/LPS/LNMMA for 12 h or 24 h. Viability was measured by MTT assay. Cell viability is expressed as percentage of viable cells with respect to control cells (100%). Results are means ± s.e.m of three independent experiments. The effect of NO donor SNAP and its structurally related non-releasing compound NAP is also shown.