Validation of DNA repair gene expression modulation following 10 Gy IR using qRT-PCR.

<p>Ct values were normalized to <i>PGK</i>. Each bar represents data from 12 different cell lines for both LCL (A) and primary fibroblasts (B) with the following exceptions: 6 samples were used for <i>PCNA</i> and <i>RRM2B</i> in the LCL experiments; 10 samples were used for <i>XPC</i>, <i>RRM2B</i>, <i>REV3L</i> for the fibroblast experiments and 5 samples for, <i>EXO1</i>, <i>PALB2</i>, <i>LIG1</i> and <i>H2AFX</i> were used in the fibroblast experiments. Gene expression levels were averaged across multiple experiments. Four separate qRT-PCR runs were carried out for <i>POLH</i>, <i>DDB2</i>, <i>APTX</i>, <i>RAD51C</i>, and <i>PALB2</i> genes; three separate qRT-PCR runs were carried out for <i>PCNA</i>, <i>REV3L</i> and <i>EXO1</i> genes; and two separate qRT-PCR runs were carried out for <i>XPC</i>, <i>RRM2B</i>, <i>H2AFX</i>, and <i>RAD51</i> genes. Error bars = SEM (n = 12). Each value on an experiment was run in triplicate. All differences shown are statistically significant (p&lt;0.05) using a t-test. PSRs used for amplification are: <i>XPC</i>: PSR853; <i>POLH</i>: PSR124; <i>DDB2</i>: PSR663; <i>PCNA</i>: PSR213; <i>RRM2B</i>: PSR293; <i>REV3L</i>: PSR729; <i>LIG1</i>: PSR905; <i>APTX</i>: PSR338; <i>H2AFX</i>: PSR185; <i>RAD51C</i>: PSR786; <i>RAD51</i>: PSR100; <i>EXO1</i>: PSR239; <i>PALB2</i>: PSR346; <i>POLL</i>: PSR904 for which some are indicated by and arrow in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053358#pone-0053358-g001" target="_blank">figures 1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053358#pone-0053358-g002" target="_blank">2</a> and all full PSR numbers can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053358#pone.0053358.s001" target="_blank">Figures S1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053358#pone.0053358.s002" target="_blank">S2</a>.