Human DNA transfer in rat colon tumors by PCR-sequencing.

<p>Representative pictures of PCR detection of a repetitive sequence of rat (<i>LINE 1</i>) in a rat tumor (DMH and DMH+SW480). (<b>A</b>) <i>Alu Yd6</i> human sequences were only amplified from the colon tumors of DMH+SW480-treated rats. Rat tail and human cells were used as positive and negative controls. Human <i>K-ras</i> and <i>RAB30</i> genes were only detected in the tumors of rats receiving DMH and SW480 cells. (<b>B</b>) Sequence analysis of the PCR product of <i>RAB30</i> in a colon tumor treated with DMH+SW480 cells. Arrows indicate the position where the nucleotide sequence is different between species and clearly shows the existence of both sequences. Human SW480 cells (control).