Expression of early activation genes is modulated by signal presentation.

<p>a) Images of CD69 stained T-cells in M− and M+ conditions. Green- CD69, Greenish black- antigen-coated beads. b) Flow cytometry analysis of CD69 and CD25 in the M− and M+ conditions at 3 and 6 hr, respectively. c) Mean fold change in CD69 and CD25 expressing cells in the M+ condition with respect to the M− condition at different time points (N = 3, mean±s.e.m.). **p<0.005. Statistical significance is calculated with respect to the M− sample at each time point. d) Mean fold change in CD69 expressing cells at 3 hr in the M+ condition (with different bead immobilizing forces) with respect to the M− condition (0 pN) (N = 3, mean±s.d.). *p<0.05, **p<0.005. Statistical significance is calculated with respect to 0pN force.