Loss of SRC-2 results in decreased expression of several cardiac transcription factors important for controlling metabolic and sarcomeric gene expression.

<p><i>A–D and F</i>, qPCR analysis of the indicated transcription factor and transcription co-activator genes. RNA was isolated from WT and SRC-2 KO hearts (n = 5). Individual gene expression is analyzed by ΔΔCt method with 18S RNA expression used as a normalizer and expression relative to WT.<i>E</i>, Immunoblot for GATA-4 and MEF2 protein expression in WT and SRC-2 KO heart tissue lysates (n = 3).* = p≤0.05, ** = p≤0.01, and *** = p≤0.001. (PPAR- peroxisome proliferator activated-receptor α, β/δ, and γ, PGC-1- peroxisome proliferator activated-receptor γ coactivator-1 α and β, SRC- steroid receptor coactivator 1 and 3, SRF- serum response factor, GATA- GATA binding protein 4 and 6, MEF2c- myocyte enhancer factor 2c, Hand 2- heart and neural crest derivatives 2, Nkx2.5- NK2 transcription factor related 5, Gfat- glutamine frustose-6-phosphate transaminase 1 and 2, Tbx5- T-box 5^#, NRF1- nuclear respiratory factor 1, ERR- estrogen-related receptor α). A # indicates a gene identified in the microarray.