Iterative antibody labelling of a BMMA embedded 2month old mouse eyelid.

<p>(A) Anti-CK1– Eyelid margin. (B) Anti-CK6– palpebral conjunctiva. (C) Anti-Ki67– nuclei of proliferating cells. (D) Anti-CK10– acidic cytokeratin which co-localises with the basic CK1 at the epidermis. (E) Reduced resolution overlay of (A, B, C, D) illustrating multiple stains (four immunolabels and one counter-stain) on the same section at a thickness not previously possible. Scale bars = 10 µm (A), 100 µm (B). OM = Obicularis Muscle, A = Acinus, CJ = Conjunctiva, MCJ = Mucocutaneous Junction, D = Duct.