Myod is required for lateral myogenesis.

<p>(A) Western blot with anti-flag and anti-tubulin antibodies of gastrula embryos injected bilaterally at the two-cell stage with 300 pg of 5utrMyf5F, 5utrMyodF or 70 pg of 5utrMrf4F synthetic mRNAs alone or with oligomorpholinos. Arrows point out the specific signal. Lane 1: synthetic mRNA alone, 2: synthetic mRNA+moControl, 3: synthetic mRNA+specific mo (moMyf5, moMyod1 or moMrf4-1 with 5utrMyf5F, 5utrMyodF or 5utrMrf4F respectively). (B) Whole-mount in situ hybridization of embryos unilaterally injected with 20 ng of moMyf5, moMyod1 or moMrf4-1 and fixed at stages 14 or 18/19. β-galactosidase mRNA (blue) was co-injected to identify the injected side, indicated by an asterisk (*). Dorsal views. The anterior side of the embryos is on the left; st., stage. (C) Transverse sections of the morphants at stage 26. (D) Transverse section of the <i>Myod</i> morphant submitted to whole-mount immunohistochemistry with the 12/101 antibody. (E) Rescue experiments: Embryos were injected unilaterally with 20 ng of mo<i>Myod</i>1 alone or co-injected with synthetic mRNA coding for MyodF (150 pg) and probed with <i>MyhE3</i>. Nc, notochord. Probes are in a framed box and indicated for each panel. For complete statistical data, see supporting information, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052359#pone.0052359.s002" target="_blank">figure S2</a>.