<p>Table shows data from a typical discrimination experiment where the three recombinant mouse isozymes have been assayed both separately and after mixing in a reconstituted sample. The four assay conditions A, B, C, and D are described both in the text and in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053271#s2" target="_blank">Methods</a>. On the right, respectively, the activity values resulting after matrix calculation and the percent errors with respect to the measured values under reference condition “A”. (<sup>b</sup>) values in brackets are the sum of individual isozyme activities.</p
<p>(A) Periodic GFP analysis was performed at indicated times of liquid cultures using FACS. GFP per...
A) MGMT activity reported as femtomoles of cleaved 32P labeled O6-MeG containing oligonucleotide per...
(A) ALE-driven workflow. (B) In vivo enzymatic comparison of wild-type Pnmt and variant shown in tim...
<p>Tissues were collected from 1 month old animals. The isozymes activities, measured by the describ...
<p>Tissues were collected from 24 month old animals. Panel A) Activity of mNMNAT isozymes from discr...
<p>A, NADS activity; B, total NMNAT activity; C, isozyme-specific NMNAT activity. Insets show in an ...
<div><p>A novel assay procedure has been developed to allow simultaneous activity discrimination in ...
A novel assay procedure has been developed to allow simultaneous activity discrimination in crude ti...
A novel assay procedure has been developed to allow simultaneous activity discrimination in crude ti...
a<p>Values are calculated for the “amidated” reaction catalyzed by NMNAT; the corresponding values f...
Enzyme specific activity (μmol/min per mg protein) measured for (A) varying malate concentrations at...
<p>A, C18-HPLC fluorescence profiles (excitation 380 nm, emission 440 nm) of pure standard pyridine ...
<p>(A) Subcellular fractionation of endogenous Nmnat3 protein in skeletal muscle. GAPDH was used as ...
<p>α-Mannosidase activity was normalized to protein and was measured from 4 mice per group. Error ba...
<p><i>Idua</i><sup>W392X</sup> mice were administered gentamicin 3-times weekly for 9 weeks without ...
<p>(A) Periodic GFP analysis was performed at indicated times of liquid cultures using FACS. GFP per...
A) MGMT activity reported as femtomoles of cleaved 32P labeled O6-MeG containing oligonucleotide per...
(A) ALE-driven workflow. (B) In vivo enzymatic comparison of wild-type Pnmt and variant shown in tim...
<p>Tissues were collected from 1 month old animals. The isozymes activities, measured by the describ...
<p>Tissues were collected from 24 month old animals. Panel A) Activity of mNMNAT isozymes from discr...
<p>A, NADS activity; B, total NMNAT activity; C, isozyme-specific NMNAT activity. Insets show in an ...
<div><p>A novel assay procedure has been developed to allow simultaneous activity discrimination in ...
A novel assay procedure has been developed to allow simultaneous activity discrimination in crude ti...
A novel assay procedure has been developed to allow simultaneous activity discrimination in crude ti...
a<p>Values are calculated for the “amidated” reaction catalyzed by NMNAT; the corresponding values f...
Enzyme specific activity (μmol/min per mg protein) measured for (A) varying malate concentrations at...
<p>A, C18-HPLC fluorescence profiles (excitation 380 nm, emission 440 nm) of pure standard pyridine ...
<p>(A) Subcellular fractionation of endogenous Nmnat3 protein in skeletal muscle. GAPDH was used as ...
<p>α-Mannosidase activity was normalized to protein and was measured from 4 mice per group. Error ba...
<p><i>Idua</i><sup>W392X</sup> mice were administered gentamicin 3-times weekly for 9 weeks without ...
<p>(A) Periodic GFP analysis was performed at indicated times of liquid cultures using FACS. GFP per...
A) MGMT activity reported as femtomoles of cleaved 32P labeled O6-MeG containing oligonucleotide per...
(A) ALE-driven workflow. (B) In vivo enzymatic comparison of wild-type Pnmt and variant shown in tim...