Tat promotes the proliferation of vIL-6 expressing cells.

<p>(<b>A</b>) Western blot analysis of Flag tagged vIL-6 and Tat expression in 4E3 and E6 cells transduced by different MOI of lentiviral Tat. (<b>B</b>) MTT assay for the proliferation of fibroblasts derived 4E3, 3D10 and control T/V cells and (<b>C</b>) endothelial cells derived E6, F7 and control E/V cells transduced by MOI 10 of Tat at days 2 to 8 post-transduction. The results represent the mean ± SD; shown is one representative experiment performed in triplicate. (<b>D</b>) MTT assay for the proliferation of fibroblasts derived 4E3, 3D10 and control T/V cells and (<b>E</b>) endothelial cells derived E6, F7 and control T/V cells stimulated with the soluble Tat (300 ng/mL) at days 1 to 3 post-treatment. **<i>P</i><.01 and ***<i>P</i><.001 for Student’s <i>t</i>-test versus corresponding controls, respectively.