Gleevec-treated cells are more adhesive than control cells.

<p>A) A schematic figure (top panel) showing the measurement of cell adhesion strength using a laminar flow system. As the laminar flow rate is increased, more cells detach. Cells were cultured for 4 hours on 10 µg/ml pre-coated collagen Nunc SlideFlask (Thermo) substrates. The fraction of adherent cells remaining after exposure to shear stress of 200 dynes/cm² for 1 min (N = 5; n = 11–20 images per N) is shown in the horizontal bar graph The group of cells treated with 20 µM of Gleevec has significantly higher number of remaining cells after laminar flow exposure (* indicates p<0.05 by the students t-test). B) and C) are representative GFP-Paxillin TIRF images for control NBT-II cells and Gleevec-treated NBT-II cells, respectively. Scale bars are 20 µm. D) and E) The average number of adhesions (D) in control and Gleevec-treated cells and the average total area of the adhesions (E) (* indicates p<0.01 by the students t-test). Error bars indicate standard deviations. Cell counts (n) are listed in the figure.