Punctuate adhesions are present at the leading edge of Gleevec-treated NBT-II cells.

<p>Panel A) and E) are representative interference reflection images of a migrating control (A) and a Gleevec-treated (E) NBT-II cell, respectively. Dense, dynamic, punctuate adhesions are only observed at the leading edge of the Gleevec-treated cells. Red rectangles on (A) and (E) show the position where thee times magnified images (B) and (F) were taken. Panel C) and G) are representative TIRF images of GFP-Paxillin expressed in control and Gleevec-treated NBT-II cells, respectively. D) and H) Images resulting from amplifying the areas indicated by the red boxes in C) and G) by three times, respectively. Gleevec-treated cells form a band of GFP-Paxillin at the leading edge of the cell. The GFP-Paxillin fluorescent intensity is measured along yellow dotted lines (shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052233#pone-0052233-g005" target="_blank">Figure 5C and 5G</a>) across the leading edge of the cell (4 lines for each cell). I) Multiple cells were used to calculate the distribution of normalized GFP-Paxillin intensity at the leading edge: control NBT-II cells (Black line, n = 12), and D-shaped Gleevec-treated NBT-II cells (red line, n = 12), with the standard deviation shown as gray (for control cells) or pink bars (for Gleevec-treated cells) (detailed calculations are described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052233#s4" target="_blank">Materials and Methods</a>). Gleevec-treated cells NBT-II cells have peak of GFP-Paxillin signal near the leading edge, while control NBT-II cells do not. Scale bars in panels A, C, E and G are 20 µm, and in B, D, F, H are 5 µm. Error bars indicate standard deviations.