Experimental validation of predictions.

<p>A) Genes predicted to be annotated with “peptidoglycan-based cell wall biogenesis,” B) genes predicted to be annotated with “translation,” C) genes predicted to be annotated with “DNA damage response,” D) genes predicted to be annotated with both “translation” and “DNA damage response,” and E) a gene predicted to be annotated with both “translation” and “ peptidoglycan-based cell wall biogenesis.” The x-axis denotes the <i>Escherichia coli</i> knockout mutant. The y-axis represents the percentage of survival of the mutant strain normalized to the wild type. Coloured bars represent the survival when the antibiotic disrupts the biological process we predict for the genes; here, the correctly annotated mutants are expected to survive less than the wild type (w. t.). Coloured lines represent the survival when we predict no effect of the antibiotic on the survival rate; here, deletion mutants were expected not to differ from the wild type. Error bars show the variation in the results among the four replicates.