Delayed entry into meiosis in XX and XY gonads lacking <i>Insr</i> and <i>Igf1r</i>.

<p>Expression of germ cell markers was assessed in XX or XY control or dko gonads either by whole mount <i>in situ</i> hybridization at E13.5 (A,B), or with double immunofluorescence at E12.5, E13.5 and E16.5 using the meiotic marker SCP3 (green) along with pluripotency marker OCT4 (red; C–N). Note the absence of SCP3 positive cells reflecting delayed progression in meiosis in XX and XY dko gonads at E13.5 (F,L). Arrows in inset H and N point toward few remaining OCT4⁺ germ cells.