Steady-state levels of mtDNA and mitochondrial transcripts.

<p>(A) Schematic linearized map of the mitochondrial genome of <i>Drosophila melanogaster</i>. (B) QRT-PCR analysis of steady-state levels of mitochondrial mRNAs in control and <i>DmMterf3</i> KD larvae at 3, 5 and 6 days ael. The analysis was normalized to levels of the transcript encoding the nuclear ribosomal protein 49. (C) Northern blot analysis of steady-state levels of mitochondrial rRNAs and mRNAs from control and <i>DmMterf3</i> KD larvae 6 days ael. Loading of gels was normalized to the transcript encoding the nuclear ribosomal protein 49. (D) Northern blot analysis of steady-state levels of mitochondrial tRNAs in control and <i>DmMterf3</i> KD larvae 6 days ael. (E) Quantification of steady-state levels of mitochondrial rRNAs and mRNAs in control and <i>DmMterf3</i> KD larvae at 6 days ael. Loading of gels was normalized to the transcript encoding the nuclear ribosomal protein 49. (F) Quantification of steady-state levels of mitochondrial tRNAs in control and <i>DmMterf3</i> KD larvae at 6 days ael. Error bars indicate mean ± SEM (^*p<0.05; ^**p<0.01; ^***p<0.001. n = 4–5).