<i>In vitro</i> splicing analysis of human HAS1 minigene.

<p>Constructs FLc and G345 are shown in (A). Arrows show where PCR primers bind (E3, E5 and E5I4). The length of each intron in G345 is shown in bp. Each construct was transfected into HeLa cells and HAS1 splicing was studied by RT-PCR. Using E3/E5 primer set, products were analyzed by agarose gel electrophoresis (B). For E3/E5I4 primer set, amplicons were analyzed by DNA fragment analysis (C). Splice junctions for each product are also illustrated. Ø, mock transfection; β2m, control.