CD44 expression in astrocyte-lineage cells during postnatal development. A–I:

<p>Double immunostaining of CD44 and GLAST in the cerebellum at P3 (A–C) and P7 (D–F). <b>G–I:</b> High magnification of D–F. <b>J–U:</b> Double immunostaining of CD44 and GFAP in the mouse cerebellum at P3 (J–L) and P7 (M–O), and at P14 in the Purkinje cell layer (P–R) and white matter (S–U). Nucleus was counterstained with TO-PRO-3 (blue). <b>V:</b> Quantitative analysis of the number of CD44-positive astrocyte-lineage cells by FACS at P3, P7 and P10. *p<0.05, **p<0.005. Scale bars, 50 µm.