Three-set Venn diagram analysis of multiple oxygen tension-response transcriptional alterations in astrocytes.

<p>(A) 3-set Venn diagram drawn by Venny. 1%, 4%, 9% represent 1%, 4%, 9% O₂ conditions, compared to the 20% O₂ condition. (B) 3-set Venn diagram generated by VennPlex. The numbers in each section show the number and type (up-, down- or contra-regulated) transcriptional alterations. As stated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053388#pone-0053388-g001" target="_blank">Figure 1</a> the boxed key helps indicate the number of factors in each set or intersection that are upregulated (<i>italic</i>), downregulated (<u>underlined</u>) or contra-regulated (red). For example, there are 60 transcripts common to all three sets, 27 are upregulated (<i>italic</i>) in all three sets, 23 are downregulated (<u>underlined</u>) in all three sets, and 10 (red) demonstrate a mixture of regulation polarities (contra-regulated) between two of the input sets.