Improved extraction methods preserve histone phosphorylation.

<p>A) Coomassie-stained gel demonstrating the purity of extracted histone sample by acid extraction protocol. The high-salt extraction protocol yields similar high purity sample (data not shown). B) Western blot analysis performed on acid extracted histone with commercially available antibodies against H3 core, H3S10ph, H3T11ph, and H3S28ph modifications. These antibodies yielded a single band corresponding to the expected size of histone H3 (∼17 kDa) when developed with Super Signal West FEMTO Chemiluminescent Substrate.