Di-methyl H3–K4 levels in <i>meis1</i> promoter chromatin.

<p>Chromatin immunoprecipitation (ChIP) analysis of the <i>gfi1b</i> promoter and two <i>meis1</i> promoter segments as indicated <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053666#pone-0053666-g002" target="_blank">Figure 2A</a> in erythroid and megakaryocytic lineages. Relative enrichment of the indicated promoter sequences for di-methyl H3–K4 in control (scrambled) versus LSD1 knocked down cells was calculated relative to that for immunoglobulin switch µ (Sµ) sequences in MEL (A) and L8057 (B) cells respectively. Results shown are the average (solid bars) and standard deviations (error bars) of three independent experiments.