HGF induced the migration of human chondrosarcoma cells.

<p>(A) Immunohistochemistry showing HGF expression in normal cartilage and chondrosarcoma tissues (arrow shows HGF staining). Quantitative data are shown in the lower panel. (B) Quantitative PCR analysis for HGF mRNA expression in normal cartilage, normal bone, and chondrosarcoma tissues. (C and D) Cells were incubated with HGF (3–30 ng/mL), and <i>in vitro</i> migration and invasion was measured by Transwell assay after 24 h. (E) JJ012 cells were treated with HGF for 24 h, after which the wound-scratching assay was performed. Results are expressed as mean ± S.E. *<i>p</i><0.05 compared with control; #<i>p</i><0.05 compared with HGF-treated group.