PI3K-C2β and p110γ play distinct role in regulation of S1P-dependent cell migration.

<p>Random motility of HUVEC transfected with the indicated siRNAs was monitored as described in the Materials and Methods section. (A) Representative track paths throughout time of 6 cells are shown (8 h). (B) Data indicate the mean velocity/min and are means ± SEM from 3 independent experiments. **p<0.01 vs cells transfected with scrambled siRNA. (C) Results from Transwell assays performed in HUVEC transfected with the indicated siRNAs. Data are expressed as percentage of migration of cells transfected with scrambled siRNA and unstimulated (control) and are means ± SEM from 3 independent experiments. *p<0.01. Downregulation of PI3K-C2β and p110γ was confirmed by Western blotting.