The effect of androgen receptor status on the response to changing sterol status.

<p>Cells were plated in their basal media: Medium A (LNCaP), supplemented with 10 nM testosterone (305) or 10 μM casodex (364). Cells were starved overnight in Medium C, and then treated for 6 h with 25-hydroxycholesterol (25-HC) in Medium C, at the concentrations indicated. Following treatment, RNA was harvested and <i>LDLR</i>, <i>HMGCR</i>, and <i>ABCG1</i> levels were determined by qRT-PCR, normalised to the vehicle condition within each cell-line. Data presented as mean ± S.E., from three separate experiments per cell-line, each performed with triplicate wells per condition.