A–D: Western blot analyses revealed posttranslational modification of eIF5A and up regulation of TGM2 after treatment with IM.

<p>(<b>A</b>) Enlarged regions from a coomassie stained 2D-PAGE from Ba/F3-p210 cells after treatment with IM or DMSO as a control. The arrows indicate two spots for eIF5A, one at pI of 5.2 and the other one at a pI of 6.1. The latter appeared after IM treatment. (<b>B</b>) 2D-WB validated the appearance of a second spot for eIF5A at a pI of 6.2 after IM treatment. (<b>C</b>) Enlarged regions from a coomassie stained 2D-PAGE from Ba/F3-p210 cells after treatment with IM or DMSO as a control. One spot for TGM2 (arrow) demonstrated an increased expression after IM treatment. (<b>D</b>) The increased expression of TGM2 after treatment with rising concentrations of IM could be validated in human Bcr-Abl K562 cells.