Flow cytometric analysis of cocultures.

<p>N16961 and GFP labeled O395 were grown separately for 24 hours, mixed (1∶1) and samples were removed from the cocultures for flow cytometric analyses at 24 hours (A), 48 hours (B) and 7 days (C) after mixing. D. The GFP labeled (b) and non- labeled cells (c) from the cocultures were separated by FACS at the time of mixing (0 h) and 24 hour after mixing, and CFU of each population was determined. O395 monocultures (a) were used as an experimental control. Plating efficiency was scored as CFU per particle sorted from each gated population. Data is represented as means ± SD, n = 3.