Lung C3a and C5a levels are up regulated during influenza infection by exclusive contribution from CD103⁺ DCs.

<p>(<b>A</b>) Bar graph shows the amounts of C3a and C5a per 100 µg protein in BAL fluid from naïve and PR8 infected WT mice on day 2, 4 and 7 post infection. (<b>B</b> and <b>C</b>) Bar graphs showing the relative mRNA expression levels of C3 and C5 in FACS sorted lung CD103⁺ DC and CD11b⁺ DC subsets in naïve or PR8 infected WT mice on day 1 and 3 post infection. Highest expression level in the naïve mice is taken as 100%. (<b>D</b> and <b>E</b>) Bar graphs show the amount of C3a and C5a per 100 µg protein in BAL fluid from naïve and PR8 infected langerin DTR mice with or without the depletion of CD103⁺ DCs. The data are representative of three different experiments with similar results. The values are expressed as mean ± SEM.